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Texas Instruments dmd
a, Optical layout of CAPS. A galvanometer scans the excitation light sheet along the detection axis while <t>an</t> <t>ETL</t> synchronously shifts the detection focal plane; bidirectional sweeps are used to maximize volume rate. Light reflected from the “on” micromirrors is collected by the camera. b, Timing synchronization. The sCMOS camera acts as the master clock; each exposure-start trigger initiates a deterministic <t>DMD</t> mask cycle (slave) within the rolling-shutter exposure window. c, CAPS processing pipeline that comprises four main steps: DMD-coded volumetric scanning, rolling-shutter capture, PnP-ADMM reconstruction, and image reassembly to form the reconstructed volume (i–vii). (i) During continuous axial scanning, the sweep is discretized into plane groups z i (and z i+1 ), each containing CR axial planes z i,r , and fluorescence within each group is encoded by a sequential DMD mask cycle (m 1 to m CR ). (ii) Rolling-shutter capture produces staggered line exposure; ordered line segments are denoted LS 1 to LS CR (earliest to latest exposure timing). (iii) The resulting line-dependent mixtures are represented as plane groups v i (and v i+1 ), with line-to-time mapping determined by the calibrated rolling-shutter timing relative to DMD mask timestamps. (iv) These multiplexed mixtures are integrated into raw camera frames b i (and b i+1 ). (v) For each exposure, PnP-ADMM reconstruction solves b i = Av i + η to recover v̂ i from b i , including alternating data-fidelity, prior, and dual updates. (vi) The reconstructed plane groups v̂ i and v̂ i+1 retain the line-wise rolling-shutter timing signature. (vii) Using calibrated line timing, line segments from v̂ i and v̂ i+1 are reassembled to restore the plane groups ẑ i and ẑ i+1 .
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Images

1) Product Images from "Compressive axial-integrated planar scanning (CAPS) microscopy for high-speed volumetric imaging of cardiac dynamics"

Article Title: Compressive axial-integrated planar scanning (CAPS) microscopy for high-speed volumetric imaging of cardiac dynamics

Journal: bioRxiv

doi: 10.64898/2026.04.21.720045

a, Optical layout of CAPS. A galvanometer scans the excitation light sheet along the detection axis while an ETL synchronously shifts the detection focal plane; bidirectional sweeps are used to maximize volume rate. Light reflected from the “on” micromirrors is collected by the camera. b, Timing synchronization. The sCMOS camera acts as the master clock; each exposure-start trigger initiates a deterministic DMD mask cycle (slave) within the rolling-shutter exposure window. c, CAPS processing pipeline that comprises four main steps: DMD-coded volumetric scanning, rolling-shutter capture, PnP-ADMM reconstruction, and image reassembly to form the reconstructed volume (i–vii). (i) During continuous axial scanning, the sweep is discretized into plane groups z i (and z i+1 ), each containing CR axial planes z i,r , and fluorescence within each group is encoded by a sequential DMD mask cycle (m 1 to m CR ). (ii) Rolling-shutter capture produces staggered line exposure; ordered line segments are denoted LS 1 to LS CR (earliest to latest exposure timing). (iii) The resulting line-dependent mixtures are represented as plane groups v i (and v i+1 ), with line-to-time mapping determined by the calibrated rolling-shutter timing relative to DMD mask timestamps. (iv) These multiplexed mixtures are integrated into raw camera frames b i (and b i+1 ). (v) For each exposure, PnP-ADMM reconstruction solves b i = Av i + η to recover v̂ i from b i , including alternating data-fidelity, prior, and dual updates. (vi) The reconstructed plane groups v̂ i and v̂ i+1 retain the line-wise rolling-shutter timing signature. (vii) Using calibrated line timing, line segments from v̂ i and v̂ i+1 are reassembled to restore the plane groups ẑ i and ẑ i+1 .
Figure Legend Snippet: a, Optical layout of CAPS. A galvanometer scans the excitation light sheet along the detection axis while an ETL synchronously shifts the detection focal plane; bidirectional sweeps are used to maximize volume rate. Light reflected from the “on” micromirrors is collected by the camera. b, Timing synchronization. The sCMOS camera acts as the master clock; each exposure-start trigger initiates a deterministic DMD mask cycle (slave) within the rolling-shutter exposure window. c, CAPS processing pipeline that comprises four main steps: DMD-coded volumetric scanning, rolling-shutter capture, PnP-ADMM reconstruction, and image reassembly to form the reconstructed volume (i–vii). (i) During continuous axial scanning, the sweep is discretized into plane groups z i (and z i+1 ), each containing CR axial planes z i,r , and fluorescence within each group is encoded by a sequential DMD mask cycle (m 1 to m CR ). (ii) Rolling-shutter capture produces staggered line exposure; ordered line segments are denoted LS 1 to LS CR (earliest to latest exposure timing). (iii) The resulting line-dependent mixtures are represented as plane groups v i (and v i+1 ), with line-to-time mapping determined by the calibrated rolling-shutter timing relative to DMD mask timestamps. (iv) These multiplexed mixtures are integrated into raw camera frames b i (and b i+1 ). (v) For each exposure, PnP-ADMM reconstruction solves b i = Av i + η to recover v̂ i from b i , including alternating data-fidelity, prior, and dual updates. (vi) The reconstructed plane groups v̂ i and v̂ i+1 retain the line-wise rolling-shutter timing signature. (vii) Using calibrated line timing, line segments from v̂ i and v̂ i+1 are reassembled to restore the plane groups ẑ i and ẑ i+1 .

Techniques Used: Fluorescence



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a, Optical layout of CAPS. A galvanometer scans the excitation light sheet along the detection axis while <t>an</t> <t>ETL</t> synchronously shifts the detection focal plane; bidirectional sweeps are used to maximize volume rate. Light reflected from the “on” micromirrors is collected by the camera. b, Timing synchronization. The sCMOS camera acts as the master clock; each exposure-start trigger initiates a deterministic <t>DMD</t> mask cycle (slave) within the rolling-shutter exposure window. c, CAPS processing pipeline that comprises four main steps: DMD-coded volumetric scanning, rolling-shutter capture, PnP-ADMM reconstruction, and image reassembly to form the reconstructed volume (i–vii). (i) During continuous axial scanning, the sweep is discretized into plane groups z i (and z i+1 ), each containing CR axial planes z i,r , and fluorescence within each group is encoded by a sequential DMD mask cycle (m 1 to m CR ). (ii) Rolling-shutter capture produces staggered line exposure; ordered line segments are denoted LS 1 to LS CR (earliest to latest exposure timing). (iii) The resulting line-dependent mixtures are represented as plane groups v i (and v i+1 ), with line-to-time mapping determined by the calibrated rolling-shutter timing relative to DMD mask timestamps. (iv) These multiplexed mixtures are integrated into raw camera frames b i (and b i+1 ). (v) For each exposure, PnP-ADMM reconstruction solves b i = Av i + η to recover v̂ i from b i , including alternating data-fidelity, prior, and dual updates. (vi) The reconstructed plane groups v̂ i and v̂ i+1 retain the line-wise rolling-shutter timing signature. (vii) Using calibrated line timing, line segments from v̂ i and v̂ i+1 are reassembled to restore the plane groups ẑ i and ẑ i+1 .
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a, Optical layout of CAPS. A galvanometer scans the excitation light sheet along the detection axis while <t>an</t> <t>ETL</t> synchronously shifts the detection focal plane; bidirectional sweeps are used to maximize volume rate. Light reflected from the “on” micromirrors is collected by the camera. b, Timing synchronization. The sCMOS camera acts as the master clock; each exposure-start trigger initiates a deterministic <t>DMD</t> mask cycle (slave) within the rolling-shutter exposure window. c, CAPS processing pipeline that comprises four main steps: DMD-coded volumetric scanning, rolling-shutter capture, PnP-ADMM reconstruction, and image reassembly to form the reconstructed volume (i–vii). (i) During continuous axial scanning, the sweep is discretized into plane groups z i (and z i+1 ), each containing CR axial planes z i,r , and fluorescence within each group is encoded by a sequential DMD mask cycle (m 1 to m CR ). (ii) Rolling-shutter capture produces staggered line exposure; ordered line segments are denoted LS 1 to LS CR (earliest to latest exposure timing). (iii) The resulting line-dependent mixtures are represented as plane groups v i (and v i+1 ), with line-to-time mapping determined by the calibrated rolling-shutter timing relative to DMD mask timestamps. (iv) These multiplexed mixtures are integrated into raw camera frames b i (and b i+1 ). (v) For each exposure, PnP-ADMM reconstruction solves b i = Av i + η to recover v̂ i from b i , including alternating data-fidelity, prior, and dual updates. (vi) The reconstructed plane groups v̂ i and v̂ i+1 retain the line-wise rolling-shutter timing signature. (vii) Using calibrated line timing, line segments from v̂ i and v̂ i+1 are reassembled to restore the plane groups ẑ i and ẑ i+1 .
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a, Optical layout of CAPS. A galvanometer scans the excitation light sheet along the detection axis while <t>an</t> <t>ETL</t> synchronously shifts the detection focal plane; bidirectional sweeps are used to maximize volume rate. Light reflected from the “on” micromirrors is collected by the camera. b, Timing synchronization. The sCMOS camera acts as the master clock; each exposure-start trigger initiates a deterministic <t>DMD</t> mask cycle (slave) within the rolling-shutter exposure window. c, CAPS processing pipeline that comprises four main steps: DMD-coded volumetric scanning, rolling-shutter capture, PnP-ADMM reconstruction, and image reassembly to form the reconstructed volume (i–vii). (i) During continuous axial scanning, the sweep is discretized into plane groups z i (and z i+1 ), each containing CR axial planes z i,r , and fluorescence within each group is encoded by a sequential DMD mask cycle (m 1 to m CR ). (ii) Rolling-shutter capture produces staggered line exposure; ordered line segments are denoted LS 1 to LS CR (earliest to latest exposure timing). (iii) The resulting line-dependent mixtures are represented as plane groups v i (and v i+1 ), with line-to-time mapping determined by the calibrated rolling-shutter timing relative to DMD mask timestamps. (iv) These multiplexed mixtures are integrated into raw camera frames b i (and b i+1 ). (v) For each exposure, PnP-ADMM reconstruction solves b i = Av i + η to recover v̂ i from b i , including alternating data-fidelity, prior, and dual updates. (vi) The reconstructed plane groups v̂ i and v̂ i+1 retain the line-wise rolling-shutter timing signature. (vii) Using calibrated line timing, line segments from v̂ i and v̂ i+1 are reassembled to restore the plane groups ẑ i and ẑ i+1 .
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Image Search Results


Dark-adapted (DA) and light-adapted (LA) flash ERGs in WT and mdx 2Cv mice. A Averaged DA waveforms with OPs removed in WT (thin traces) and mdx 2Cv mice (bold traces). Flash strength and definitions of key components (a-wave, b-wave) are indicated. B Mean (± SD) amplitudes (μV; upper plots) and implicit times (ms; lower plots) of DA a- and b-waves as a function of flash strength, in WT (open symbols) and mdx 2Cv mice (filled symbols). C Averaged OP traces isolated from the strongest scotopic flash (0.3 log cd.s/m 2 ) in WT (thin traces) and mdx 2Cv mice (bold traces). D Mean (± SD) amplitude of OPs as a function of flash strength in WT (open symbols) and mdx 2Cv (filled symbols) mice. E Averaged LA waveforms of WT (thin traces) and mdx 2Cv mice (bold traces) at 0.3 log cd.s/m 2 flash strength. F Histograms showing the mean (± SD) amplitude and implicit time of the LA b-wave. Dotted lines in A, C and E show the physiological hallmarks used for measurement of amplitudes and/or implicit times; the light-grey arrowhead marks the onset of the stimulus. Recordings made in 32 eyes of 16 WT and 32 eyes of 16 mdx 2Cv mice. Significant genotype effects ( p < 0.05 ) are marked with an asterisk

Journal: BMC Medicine

Article Title: Dystrophin-gene mutation location influences severity of electroretinogram defects in mouse models of Duchenne muscular dystrophy

doi: 10.1186/s12916-026-04873-1

Figure Lengend Snippet: Dark-adapted (DA) and light-adapted (LA) flash ERGs in WT and mdx 2Cv mice. A Averaged DA waveforms with OPs removed in WT (thin traces) and mdx 2Cv mice (bold traces). Flash strength and definitions of key components (a-wave, b-wave) are indicated. B Mean (± SD) amplitudes (μV; upper plots) and implicit times (ms; lower plots) of DA a- and b-waves as a function of flash strength, in WT (open symbols) and mdx 2Cv mice (filled symbols). C Averaged OP traces isolated from the strongest scotopic flash (0.3 log cd.s/m 2 ) in WT (thin traces) and mdx 2Cv mice (bold traces). D Mean (± SD) amplitude of OPs as a function of flash strength in WT (open symbols) and mdx 2Cv (filled symbols) mice. E Averaged LA waveforms of WT (thin traces) and mdx 2Cv mice (bold traces) at 0.3 log cd.s/m 2 flash strength. F Histograms showing the mean (± SD) amplitude and implicit time of the LA b-wave. Dotted lines in A, C and E show the physiological hallmarks used for measurement of amplitudes and/or implicit times; the light-grey arrowhead marks the onset of the stimulus. Recordings made in 32 eyes of 16 WT and 32 eyes of 16 mdx 2Cv mice. Significant genotype effects ( p < 0.05 ) are marked with an asterisk

Article Snippet: Breeders (B6Ros.Cg-Dmd mdx−2Cv ) were purchased from the Jackson Laboratory (JAX stock #002388; Bar Harbor, ME, USA).

Techniques: Isolation

Relative changes of Dark-adapted (DA) and light-adapted (LA) flash ERGs in DMD mouse models. A Average DA waveforms with OPs removed in mdx (blue traces), mdx 2Cv (orange traces), mdx52 (gray traces) and dmd-null mice (yellow traces). Flash strength and definitions of key components (a-wave, b-wave) are indicated. B Histograms showing the mean (± SEM) normalized amplitudes (% of WT; upper plots) and the difference between WT and mutant implicit times (ms; lower plots) for DA a- and b-waves, in mdx (blue), mdx 2Cv (orange), mdx52 (gray) and dmd-null (yellow) mice. C Averaged OP traces isolated from the strongest scotopic flash (0.3 log cd.s/m 2 ) in mdx (blue), mdx 2Cv (orange), mdx52 (gray) and dmd-null mice (yellow). D Histograms showing the mean (± SEM) normalized amplitude of OPs in mdx (blue), mdx 2Cv (orange), mdx52 (gray) and dmd-null (yellow) mice. E Averaged LA waveforms of mdx (blue), mdx 2Cv (orange), mdx52 (gray) and dmd-null (yellow). F Histograms showing the mean (± SD) amplitude and implicit time of the LA b-wave. The red dotted lines on histograms represents the control (WT). Dotted lines in A, C and E show the physiological hallmarks used for measurement of amplitudes and/or implicit times; the light-grey arrowhead marks the onset of the stimulus. Recordings were made in 34 eyes of mdx , 34 eyes of mdx 2Cv , 20 eyes of mdx52 and 28 eyes of dmd-null mice. Significant differences from WT littermate means are marked with a # ( p < 0.05 ) in the bar plot; significant differences between DMD mouse lines are marked with an asterisk ( p < 0.05 ). NS: not significant

Journal: BMC Medicine

Article Title: Dystrophin-gene mutation location influences severity of electroretinogram defects in mouse models of Duchenne muscular dystrophy

doi: 10.1186/s12916-026-04873-1

Figure Lengend Snippet: Relative changes of Dark-adapted (DA) and light-adapted (LA) flash ERGs in DMD mouse models. A Average DA waveforms with OPs removed in mdx (blue traces), mdx 2Cv (orange traces), mdx52 (gray traces) and dmd-null mice (yellow traces). Flash strength and definitions of key components (a-wave, b-wave) are indicated. B Histograms showing the mean (± SEM) normalized amplitudes (% of WT; upper plots) and the difference between WT and mutant implicit times (ms; lower plots) for DA a- and b-waves, in mdx (blue), mdx 2Cv (orange), mdx52 (gray) and dmd-null (yellow) mice. C Averaged OP traces isolated from the strongest scotopic flash (0.3 log cd.s/m 2 ) in mdx (blue), mdx 2Cv (orange), mdx52 (gray) and dmd-null mice (yellow). D Histograms showing the mean (± SEM) normalized amplitude of OPs in mdx (blue), mdx 2Cv (orange), mdx52 (gray) and dmd-null (yellow) mice. E Averaged LA waveforms of mdx (blue), mdx 2Cv (orange), mdx52 (gray) and dmd-null (yellow). F Histograms showing the mean (± SD) amplitude and implicit time of the LA b-wave. The red dotted lines on histograms represents the control (WT). Dotted lines in A, C and E show the physiological hallmarks used for measurement of amplitudes and/or implicit times; the light-grey arrowhead marks the onset of the stimulus. Recordings were made in 34 eyes of mdx , 34 eyes of mdx 2Cv , 20 eyes of mdx52 and 28 eyes of dmd-null mice. Significant differences from WT littermate means are marked with a # ( p < 0.05 ) in the bar plot; significant differences between DMD mouse lines are marked with an asterisk ( p < 0.05 ). NS: not significant

Article Snippet: Breeders (B6Ros.Cg-Dmd mdx−2Cv ) were purchased from the Jackson Laboratory (JAX stock #002388; Bar Harbor, ME, USA).

Techniques: Mutagenesis, Isolation, Control

a, Optical layout of CAPS. A galvanometer scans the excitation light sheet along the detection axis while an ETL synchronously shifts the detection focal plane; bidirectional sweeps are used to maximize volume rate. Light reflected from the “on” micromirrors is collected by the camera. b, Timing synchronization. The sCMOS camera acts as the master clock; each exposure-start trigger initiates a deterministic DMD mask cycle (slave) within the rolling-shutter exposure window. c, CAPS processing pipeline that comprises four main steps: DMD-coded volumetric scanning, rolling-shutter capture, PnP-ADMM reconstruction, and image reassembly to form the reconstructed volume (i–vii). (i) During continuous axial scanning, the sweep is discretized into plane groups z i (and z i+1 ), each containing CR axial planes z i,r , and fluorescence within each group is encoded by a sequential DMD mask cycle (m 1 to m CR ). (ii) Rolling-shutter capture produces staggered line exposure; ordered line segments are denoted LS 1 to LS CR (earliest to latest exposure timing). (iii) The resulting line-dependent mixtures are represented as plane groups v i (and v i+1 ), with line-to-time mapping determined by the calibrated rolling-shutter timing relative to DMD mask timestamps. (iv) These multiplexed mixtures are integrated into raw camera frames b i (and b i+1 ). (v) For each exposure, PnP-ADMM reconstruction solves b i = Av i + η to recover v̂ i from b i , including alternating data-fidelity, prior, and dual updates. (vi) The reconstructed plane groups v̂ i and v̂ i+1 retain the line-wise rolling-shutter timing signature. (vii) Using calibrated line timing, line segments from v̂ i and v̂ i+1 are reassembled to restore the plane groups ẑ i and ẑ i+1 .

Journal: bioRxiv

Article Title: Compressive axial-integrated planar scanning (CAPS) microscopy for high-speed volumetric imaging of cardiac dynamics

doi: 10.64898/2026.04.21.720045

Figure Lengend Snippet: a, Optical layout of CAPS. A galvanometer scans the excitation light sheet along the detection axis while an ETL synchronously shifts the detection focal plane; bidirectional sweeps are used to maximize volume rate. Light reflected from the “on” micromirrors is collected by the camera. b, Timing synchronization. The sCMOS camera acts as the master clock; each exposure-start trigger initiates a deterministic DMD mask cycle (slave) within the rolling-shutter exposure window. c, CAPS processing pipeline that comprises four main steps: DMD-coded volumetric scanning, rolling-shutter capture, PnP-ADMM reconstruction, and image reassembly to form the reconstructed volume (i–vii). (i) During continuous axial scanning, the sweep is discretized into plane groups z i (and z i+1 ), each containing CR axial planes z i,r , and fluorescence within each group is encoded by a sequential DMD mask cycle (m 1 to m CR ). (ii) Rolling-shutter capture produces staggered line exposure; ordered line segments are denoted LS 1 to LS CR (earliest to latest exposure timing). (iii) The resulting line-dependent mixtures are represented as plane groups v i (and v i+1 ), with line-to-time mapping determined by the calibrated rolling-shutter timing relative to DMD mask timestamps. (iv) These multiplexed mixtures are integrated into raw camera frames b i (and b i+1 ). (v) For each exposure, PnP-ADMM reconstruction solves b i = Av i + η to recover v̂ i from b i , including alternating data-fidelity, prior, and dual updates. (vi) The reconstructed plane groups v̂ i and v̂ i+1 retain the line-wise rolling-shutter timing signature. (vii) Using calibrated line timing, line segments from v̂ i and v̂ i+1 are reassembled to restore the plane groups ẑ i and ẑ i+1 .

Article Snippet: Downstream of the ETL, the second relay lens forms the image onto a DMD (DLP9500, Texas Instruments).

Techniques: Fluorescence